Restriction Enzymes
New England Biolabs, Inc. NcoI-HF – 5000 units
A high fidelity restriction endonuclease that recognizes the sequence C^CATG_G. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
ABclonal Technology SRF Rabbit pAb
This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways, for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs). Two transcript variants encoding different isoforms have been found for this gene.
New England Biolabs, Inc. FatI – 50 units
A restriction endonuclease that recognizes the sequence ^CATG_.
Sorbent Technologies Inc Silica for DCC, w/UV254, 60A, 63-200um, 500g
Silica for Dry Column Chromatography (DCC) is a fast, easy, and efficient method for separating and/or purifying industrial quantities of compounds. DCC links analytical thin layer chromatography (TLC) with preparative column chromatography (CC). Like TLC, DCC is a "non-elution" method utilizing Nylon Foil Tubing as a support. In TLC solvent flow is by capillary action going against gravity whereas in DCC, solvent flow is by gravity. Like Column Chromatography, columns of most any size may be filled in DCC to separate or clean up very large quantities of materials. Silica Gel for DCC consists of a UV254 fluorescent indicator and a silica gel with a porosity of 60A, particle distribution of 63-200um, and a quantity of 500g.